解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::HbA1c test has been widely used to evaluate glycemic control in patients with diabetes. However, there are controversial results regarding the value of HbA1c in the diagnosis of diabetes mellitus (DM). The present study investigates the diagnostic effectiveness of HbA1c in a large patient group. The oral glucose toler...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章,多中心研究
doi:10.1007/s12010-019-03080-4
更新日期:2020-01-01 00:00:00
abstract::A highly sensitive, selective, reversible, and reusable glucose sensor is developed by using molecularly imprinted polymer-based artificial receptors onto interdigital transducer. Sensor receptors were synthesized through bulk imprinting technology by using styrene as monomer, ethylene glycol dimethacrylate (EGDMA) as...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-019-03049-3
更新日期:2019-12-01 00:00:00
abstract::The dye decolorization potential of the white-rot fungus Phlebia brevispora TMIC33929 when grown alone or in coculture with its growth-promoting bacterium Enterobacter sp. TN3W-14 was evaluated in low nitrogen liquid medium at different pHs. Axenic fungus removed a similar amount of Congo red and crystal violet at pH ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-019-03062-6
更新日期:2019-12-01 00:00:00
abstract::This study investigated methane production in an anaerobic sequencing batch biofilm reactor (AnSBBR) by co-digesting sugarcane vinasse and cheese whey. The assessment was based on the influence of feed strategy, interaction between cycle time and influent concentration, applied volumetric organic load (OLRA), and temp...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-019-03056-4
更新日期:2019-11-01 00:00:00
abstract::It has been found that lactic acid and hydrogen peroxide (H2O2) displayed co-operatively enhanced killing activity to pathogens. The synergistic effect was investigated with using several microbe species, suggesting that low concentration of lactic acid and H2O2 could kill both Gram-negative and Gram-positive bacteria...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-019-03031-z
更新日期:2019-11-01 00:00:00
abstract::Aminoglycosides are widely used antibiotics that bind to the bacterial 30S ribosomal subunit to inhibit translation. Owing to their adverse side effects and narrow therapeutic index, monitoring blood levels of aminoglycosides is important to maximize their effectiveness and minimize their toxicity. Current monitoring ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-019-03048-4
更新日期:2019-11-01 00:00:00
abstract::For efficiently enhancing the activity of isoeugenol monooxygenase, a whole cell overproducing active aggregate IEM720-18A was successfully fabricated via the fusion of amphiphilic short peptide 18A (EWLKAFYEKVLEKLKELF) and isoeugenol monooxygenase and then efficiently expressed in E. coli BL21 (DE3). Such resulting b...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-019-02996-1
更新日期:2019-10-01 00:00:00
abstract::The articular cartilage is an avascular and aneural tissue and its injuries result mostly in osteoarthritic changes and formation of fibrous tissue. Efforts of scientists worldwide are focused on restoration of cartilage with increase in life quality of patients. Novel polymeric polyhydroxybutyrate/chitosan (PCH) poro...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-019-03021-1
更新日期:2019-10-01 00:00:00
abstract::Acute kidney injury (AKI) is a rapid loss of renal function. It has high mortality rates. Still, renal replacement therapy is considered the best solution for recovering AKI. This opens a line of thought to develop an alternative therapy for it without complications. Mesenchymal stem cells are considered a new therapy...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-019-02995-2
更新日期:2019-09-01 00:00:00
abstract::Ras-related domain family member 1 transcript variant A (RASSF1A) controls apoptosis and cell proliferation while p14/ARF gene has a regulatory role in cellular senescence. Failure of apoptosis and cellular senescence occurs during inflammatory bowel disease (IBD) and colorectal cancer (CRC). To reveal the role of per...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-019-03017-x
更新日期:2019-09-01 00:00:00
abstract::Agar, a major polysaccharide of red algal cells, is degraded by β-agarases into neoagarobiose, which is further hydrolyzed into the monomers, D-galactose and 3,6-anhydro-L-galactose, by 1,3-α-3,6-anhydro-L-galactosidases including α-1,3-L-neoagarooligasaccharide hydrolase (α-NAOSH). A novel cold-adapted alkaline α-NAO...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-019-02963-w
更新日期:2019-08-01 00:00:00
abstract::Lupulones, naturally produced by glandular trichomes of hop (Humulus lupulus), are prenylated phloroglucinol derivatives that contribute the bitter flavor of beer and demonstrate antimicrobial and anticancer activities. It is appealing to develop microbial cell factories such that lupulones may be produced via ferment...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-019-02957-8
更新日期:2019-07-01 00:00:00
abstract::The purpose of this study was to examine the protective effects of intracellular homogeneous melanin produced by Lachnum YM156 (LIM) against ultraviolet B (UVB) induced damage in mice. The possible structural formula of the LIM was concluded based on elemental analysis, ultraviolet-visible spectroscopy (UV-Vis), Fouri...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2898-9
更新日期:2019-06-01 00:00:00
abstract::Carnosol and carnosic acid are polyphenolic compounds found in rosemary and sage with known anti-oxidant, anti-inflammatory, and anti-microbial properties. Here, we addressed the potential use of carnosol and carnosic acid for in vitro bone tissue engineering applications, specifically depending on their cytotoxic eff...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-02934-7
更新日期:2019-06-01 00:00:00
abstract::6-Tuliposides A (6-PosA) and B (6-PosB) are major secondary metabolites in tulip (Tulipa gesneriana), having an acyl group at the C-6 position of D-glucose. They serve as precursors of the antimicrobial α-methylene-γ-butyrolactones tulipalins A (PaA) and B (PaB). The conversions of 6-PosA/6-PosB to PaA/PaB are catalyz...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2903-3
更新日期:2019-05-01 00:00:00
abstract::Sorghum was pretreated by sole ultrasound or supercritical carbon dioxide (scCO2), as well as the method combining both to intensify enzymatic hydrolysis. The effect of the time (1-5 h) and temperature (30-70 °C) on ultrasonic pretreatment was investigated, and the best condition was determined as 5 h and 50 °C with t...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2909-x
更新日期:2019-05-01 00:00:00
abstract::Vegetable and fruit waste (VFW) is becoming a heavy burden of municipal waste disposal because of its huge amount, but it is a potentially valuable resource that can be developed into high value products such as methane. Conventional anaerobic digestion processes are not suitable for solving the problem of easy acidif...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2910-4
更新日期:2019-05-01 00:00:00
abstract::Microalgae are photosynthetic microorganisms that produce several bioactive molecules that have received considerable attention in scientific and industrial communities. Today, many plant biostimulants including seaweed extracts and polysaccharides are used in agriculture. However, microalgae have not been largely exp...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2916-y
更新日期:2019-05-01 00:00:00
abstract::A 28-day feeding experiment with formulated feed using docosahexaenoic acid (DHA)-rich whole cells of freeze-dried marine microalgae Schizochytrium sp. to understand the distribution of fatty acids in a laboratory model zebrafish was conducted. Three feeds, commercial feed, 50:50 feed (50% commercial and 50% algae), a...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2906-0
更新日期:2019-05-01 00:00:00
abstract::The vegetative insecticidal proteins (VIPs) of Bacillus thuringiensis (Bt) have a broad-spectrum insecticidal activity against Lepidopteran pests and no cross-resistance with the insecticidal crystal protein Cry protein. So there are great potentials for the control of agricultural pests and the resolution of resistan...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12010-018-2880-6
更新日期:2019-04-01 00:00:00
abstract::Detection of minute quantities of target antigens in serum samples (consisting of a mixture of proteins/biomolecules) can be achieved by enhancement of the capture efficiencies of heterogeneous immunosensors. An important process parameter which affects the capture of target analytes in such immunosensors is the pH of...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2883-3
更新日期:2019-04-01 00:00:00
abstract::Lung cancer remains a highly prevalent disease and a leading cause of cancer-related deaths worldwide. Currently, exploring antitumor drugs derived from herbs used in traditional Chinese medicine is increasingly becoming an attractive area of research. Paclitaxel (PTX), a highly effective chemotherapeutic drug, is wid...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2869-1
更新日期:2019-04-01 00:00:00
abstract::This work investigated the cultivation of Chlorella vulgaris in a thin-film flat plate photobioreactor under outdoor conditions and using digested piggery wastewater as the culture medium. The algal cells were able to adapt quickly to the wastewater and outdoor conditions. A specific growth rate of 0.12 day-1 was obta...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2889-x
更新日期:2019-04-01 00:00:00
abstract::Pseudomonas are ubiquitous bacteria that can live under a wide range of environmental conditions; some species are successfully used in the fermentation industry. Pseudomonas plecoglossicida JUIM01 strain is an important industrial 2-ketogluconate (2KGA) producer. In Pseudomonas, kguT gene encodes a putative 2KGA tran...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2843-y
更新日期:2019-03-01 00:00:00
abstract::The cellulosome is a supramolecular multienzyme complex formed via species-specific interactions between the cohesin modules of scaffoldin proteins and the dockerin modules of a wide variety of polysaccharide-degrading enzymes. Here, we report a comparative analysis of cellulosomes prepared from the thermophilic anaer...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2864-6
更新日期:2019-03-01 00:00:00
abstract::Multi-strain mixed fermentation can provide a relatively complete lignocellulosic enzyme system compared with single-strain fermentation. This study was firstly to screen strains which have a strong ability to hydrolyse rice straw (RS) enzymatically and enrich true protein (TP). Then, the conditions in the process of ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2792-5
更新日期:2019-01-01 00:00:00
abstract::Graphene-containing 13-93 bioactive glass and poly(ε-caprolactone)-based bilayer, electrically conductive scaffolds were prepared for osteochondral tissue repair. Biological response of osteoblastic MC3T3-E1 and chondrogenic ATDC5 cells to the composite scaffolds was assessed under mono-culture and co-culture conditio...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2758-7
更新日期:2018-12-01 00:00:00
abstract::The aim of this work is to investigate the binding affinity between the prokaryotic proteins-AHU-IHF proteins (AHU (AHU2, TR3, and AHU6) and IHF (IHF-WT and IHF-βE44A))-and DNAs (DNA, H'-DNA, and H'44A-DNA) by using the steered molecular dynamics (SMD) simulation and the molecular mechanics Poisson-Boltzmann surface a...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2735-1
更新日期:2018-12-01 00:00:00
abstract::The first continuous production system of laminaribiose from sucrose and glucose in a bienzymatic reaction is reported in this study. Immobilized laminaribiose phosphorylase and sucrose phosphorylase were used in a packed bed reactor system comprising of a 3-cm glass column at 35 °C with a steady feeding flow rate of ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2779-2
更新日期:2018-12-01 00:00:00
abstract::This work describes the use of an ultrasound system for the enzymatic transesterification of oils using combi-lipases as biocatalyst. The reactions were carried out evaluating the individual use of waste oil and fresh soybean oil, and the immobilized lipases CALB, TLL, and RML were used as biocatalysts. It was perform...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2763-x
更新日期:2018-11-01 00:00:00
abstract::Lysozymes are known as ubiquitously distributed immune effectors with hydrolytic activity against peptidoglycan, the major bacterial cell wall polymer, to trigger cell lysis. In the present study, the full-length cDNA sequence of a novel sea urchin Strongylocentrotus purpuratus invertebrate-type lysozyme (sp-iLys) was...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2753-z
更新日期:2018-10-01 00:00:00
abstract::Since the adult mammalian heart has limited regenerative capacity, cardiac trauma, disease, and aging cause permanent loss of contractile tissue. This has fueled the development of stem cell-based strategies to provide the damaged heart with new cardiomyocytes. Bone marrow-derived mesenchymal stem cells (BM-MSCs) are ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2734-2
更新日期:2018-09-01 00:00:00
abstract::The primary structure of macrodontain I, a peptidase from Pseudananas macrodontes fruits, was determined using Edman's degradation. The enzyme is a non-glycosylated peptidase composed by 213 amino acids with a calculated molecular weight of 23,486.18 Da, pI value 6.99, and a molar extinction coefficient at 280 nm of 6...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2725-3
更新日期:2018-09-01 00:00:00
abstract::Genetic polymorphism of the cytochrome P450 (CYP) genes particularly affects CYP2D6 and CYP2C19 to a functionally relevant extent, and it is therefore crucial to elucidate the enzyme kinetic and molecular basis for altered catalytic activity of these allelic variants. This study explored the expression and function of...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2728-0
更新日期:2018-09-01 00:00:00
abstract::The coarse perlite 40-80 mesh was selected as an immobilizing material and put into a packed bed reactor (PBR) to continuously convert maltose to isomalto-oligosaccharides (IMOs). The PBR was prepared by mixing the thermo-inactivated cells (TIC) from Aspergillus niger J2 strain with the coarse perlite, then the mixtur...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2706-6
更新日期:2018-08-01 00:00:00
abstract::Forty-four endophytic fungal isolates obtained from marine sponge, Hyrtios erectus, were evaluated and screened for their hydrolase activities. Most of the isolates were found to be prolific producers of hydrolytic enzymes. Only 11 isolates exhibited maximum cellular contents of lipids, rhamnolipids, and protein in th...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-017-2679-x
更新日期:2018-07-01 00:00:00
abstract::In a recombinant antibody scFv format, the presence of an unpaired cysteine (Cys) is implicated in reduced soluble expression and inefficient presentation in phage display. Compared to other species, antibodies derived from rabbits are more likely to contain this unpaired Cys residue at position 80 (Cys80), when gener...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-017-2691-1
更新日期:2018-07-01 00:00:00
abstract::Exopolysaccharide of Lachnum YM130 (LEP) was purified by diethylaminoethyl cellulose 52 and Sepharose CL-6B column chromatography. LEP-2a was identified to be a homogeneous component with an average molecular weight of 1.31 × 106 Da, which was consisted of mannose and galactose in a molar ratio of 3.8:1.0. The structu...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-017-2668-0
更新日期:2018-06-01 00:00:00
abstract::The increasing death rates related to anaplastic lymphoma kinase (ALK)-positive lung cancer culminated in a significant interest in the discovery of novel inhibitors for ALK. In the present research work, pharmacophore-based 3D QSAR modeling and virtual screening strategy have been carried out to address these issues....
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-017-2650-x
更新日期:2018-05-01 00:00:00
abstract::Magnetosomes are specialized organelles arranged in intracellular chains in magnetotactic bacteria. The superparamagnetic property of these magnetite crystals provides potential applications as contrast-enhancing agents for magnetic resonance imaging. In this study, we compared two different nanoparticles that are bac...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-017-2642-x
更新日期:2018-05-01 00:00:00